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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488 Anti-Human CD226 (11A8) Mouse IgG2a Recombinant Antibody

CD226 Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

11A8

Cat no : CL488-65573

Synonyms

CD226, CD226 antigen, CD226 molecule, DNAM 1, DNAM1, DNAX accessory molecule 1, PTA1, TLiSA1

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using CoraLite® Plus 488 Anti-Human CD226 (11A8) Mouse I (CL488-65573)

    FC experiment of human PBMCs using CL488-65573

    1x10^6 human PBMCs were surface stained with 5 ul Coralite® Plus 488 CD226 Recombinant Antibody (CL488-65573, Clone:11A8) or CoraLite® Plus 488 Mouse IgG2a Isotype Control (C1.18.4) (CL488-65208, Clone: C1.18.4). Cells were then stained with 5 ul APC Anti-Human CD3 (OKT3) (APC-65133, Clone: OKT3). Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using CoraLite® Plus 488 Anti-Human CD226 (11A8) Mouse I (CL488-65573)

    FC experiment of human PBMCs using CL488-65573

    1x10^6 human PBMCs were surface stained with 5 ul Coralite® Plus 488 CD226 Recombinant Antibody (CL488-65573, Clone:11A8) (right) or 5 ul CoraLite® Plus 488 Anti-Human CD226 (11A8) (CL488-65247, Clone: 11A8) (left). Cells were then stained with 5 ul APC Anti-Human CD3 (OKT3) (APC-65133, Clone: OKT3). Cells were not fixed. Lymphocytes were gated.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-65573 targets CD226 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen Human NK Cells 相同性解析による交差性が予測される生物種
Full Name CD226 molecule
Calculated molecular weight 336 aa, 39 kDa
GenBank accession numberBC074787
Gene symbol CD226
Gene ID (NCBI) 10666
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD226 (DNAM-1) is a ~65 kDa glycoprotein expressed on the surface of NK cells, platelets, monocytes and a subset of T cells. It is a member of the Ig-superfamily containing 2 Ig-like domains of the V-set. CD226 mediates cellular adhesion of platelets and megakaryocytic cells to vascular endothelial cells. The protein also plays a role in megakaryocytic cell maturation. Interactions of CD226 and its ligands, CD155 and CD112, induce NK and T cell-mediated cytotoxicity and cytokine secretion (PMID: 15039383).

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 CD226 antibody CL488-65573Download protocol
Standard Protocols
Click here to view our Standard Protocols