• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 750 Anti-Human CD226 (11A8) Mouse IgG2a Recombinant Antibody

CD226 Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

11A8

Cat no : CL750-65573

Synonyms

DNAM-1, DNAM1, DNAM 1, CD226 molecule, CD226 antigen

Validation Data Gallery

Filter:
  • Flow cytometry (FC) experiment of human PBMCs using CoraLite® Plus 750 Anti-Human CD226 (11A8) Mouse I (CL750-65573)

    FC experiment of human PBMCs using CL750-65573

    1x10^6 human PBMCs were surface stained with CoraLite® Plus 488 Anti-Human CD3 and 5 ul CoraLite® Plus 750 Anti-Human CD226 (11A8) Mouse IgG2a Recombinant Antibody (CL750-65573, Clone: 11A8) (right), or CoraLite® Plus 750 Mouse IgG2a Isotype Control (C1.18.4) (CL750-65208, Clone: C1.18.4) (left). Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using CoraLite® Plus 750 Anti-Human CD226 (11A8) Mouse I (CL750-65573)

    FC experiment of human PBMCs using CL750-65573

    1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 750 Anti-Human CD226 (11A8) Mouse IgG2a Recombinant Antibody (CL750-65573, Clone: 11A8) (red), or CoraLite® Plus 750 Mouse IgG2a Isotype Control (C1.18.4) (CL750-65208, Clone: C1.18.4) (blue). Cells were not fixed. Lymphocytes were gated.

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL750-65573 targets CD226 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen Human NK Cells 相同性解析による交差性が予測される生物種
Full Name CD226 molecule
Calculated molecular weight 336 aa, 39 kDa
GenBank accession numberBC074787
Gene symbol CD226
Gene ID (NCBI) 10666
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission maxima wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD226 (DNAM-1) is a ~65 kDa glycoprotein expressed on the surface of NK cells, platelets, monocytes and a subset of T cells. It is a member of the Ig-superfamily containing 2 Ig-like domains of the V-set. CD226 mediates cellular adhesion of platelets and megakaryocytic cells to vascular endothelial cells. The protein also plays a role in megakaryocytic cell maturation. Interactions of CD226 and its ligands, CD155 and CD112, induce NK and T cell-mediated cytotoxicity and cytokine secretion (PMID: 15039383).

Protocols

Product Specific Protocols
FC protocol for CL Plus 750 CD226 antibody CL750-65573Download protocol
Standard Protocols
Click here to view our Standard Protocols