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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD3 (Hit3a)

CD3 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2a, kappa

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

Hit3a

Cat no : 65112-1-Ig

Synonyms

CD3, CD3E, T3E, TCRE

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD3 (Hit3a) (65112-1-Ig)

    FC experiment of human PBMCs using 65112-1-Ig

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD3 (65112-1-Ig, Clone:Hit3a) or Mouse IgG2a Isotype Control and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:500, then CL750 Anti-Human CD45. Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD3 (Hit3a) (65112-1-Ig)

    FC experiment of human PBMCs using 65112-1-Ig

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD3 (65112-1-Ig, Clone:Hit3a) or Mouse IgG2a Isotype Control and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:500. Cells were not fixed. Lymphocytes were gated.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65112-1-Ig targets CD3 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a, kappa
Class Monoclonal
Type Antibody
Immunogen N/A 相同性解析による交差性が予測される生物種
Full Name CD3e molecule, epsilon (CD3-TCR complex)
Calculated molecular weight 207 aa, 23 kDa
GenBank accession numberBC049847
Gene symbol CD3
Gene ID (NCBI) 916
RRIDAB_2918410
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

Protocols

Product Specific Protocols
FC protocol for CD3 antibody 65112-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols