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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 647 Anti-Human CD3 (Hit3a)

CD3 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2a, kappa

Reactivity

Human

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

Hit3a

Cat no : CL647-65112

Synonyms

CD3, CD3E, T3E, TCRE

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human peripheral blood lymphocytes using CoraLite® Plus 647 Anti-Human CD3 (Hit3a) (CL647-65112)

    FC experiment of human peripheral blood lymphocytes using CL647-65112

    1X10^6 human peripheral blood lymphocytes were surface stained with FITC-Anti-Human CD19 (FITC-65145, Clone: SJ25C1) and 5.00 ul CoraLite® Plus 647-conjugated Anti-Human CD3 (CL647-65112, Clone: Hit3a). Cells were not fixed.

  • Flow cytometry (FC) experiment of human peripheral blood lymphocytes using CoraLite® Plus 647 Anti-Human CD3 (Hit3a) (CL647-65112)

    FC experiment of human peripheral blood lymphocytes using CL647-65112

    1X10^6 human peripheral blood lymphocytes were surface stained with FITC-Anti-Human CD19 (FITC-65145, Clone: SJ25C1) and CoraLite® Plus 647-conjugated Mouse IgG2a isotype control antibody. Cells were not fixed.

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Tested Applications

Positive FC detected inhuman peripheral blood lymphocytes

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65112 targets CD3 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a, kappa
Class Monoclonal
Type Antibody
Immunogen N/A 相同性解析による交差性が予測される生物種
Full Name CD3e molecule, epsilon (CD3-TCR complex)
Calculated molecular weight 207 aa, 23 kDa
GenBank accession numberBC049847
Gene symbol CD3
Gene ID (NCBI) 916
RRIDAB_2883689
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 CD3 antibody CL647-65112Download protocol
Standard Protocols
Click here to view our Standard Protocols