FITC Plus Anti-Human CD3 (Hit3a)
CD3 Monoclonal Antibody for FC
Host / Isotype
Mouse / IgG2a, kappa
Reactivity
Human
Applications
FC
Conjugate
FITC Plus Fluorescent Dye
CloneNo.
Hit3a
Cat no : FITC-65112
Synonyms
Validation Data Gallery
Tested Applications
Positive FC detected in | human PBMCs |
Recommended dilution
Application | Dilution |
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This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
FITC-65112 targets CD3 in FC applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Mouse / IgG2a, kappa |
Class | Monoclonal |
Type | Antibody |
Immunogen | N/A 相同性解析による交差性が予測される生物種 |
Full Name | CD3e molecule, epsilon (CD3-TCR complex) |
Calculated molecular weight | 207 aa, 23 kDa |
GenBank accession number | BC049847 |
Gene symbol | CD3 |
Gene ID (NCBI) | 916 |
RRID | AB_2883783 |
Conjugate | FITC Plus Fluorescent Dye |
Excitation/Emission maxima wavelengths | 495 nm / 524 nm |
Form | Liquid |
Purification Method | Affinity purification |
Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA. |
Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).
Protocols
Product Specific Protocols | |
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FC protocol for FITC Plus CD3 antibody FITC-65112 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |