CoraLite® Plus 488 Anti-Mouse CD3ε (145-2C11)
CD3ε Monoclonal Antibody for FC
Host / Isotype
Hamster / IgG
Reactivity
Mouse
Applications
FC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
145-2C11
Cat no : CL488-65060
Synonyms
Validation Data Gallery
Tested Applications
| Positive FC detected in | mouse splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-65060 targets CD3ε in FC applications and shows reactivity with Mouse samples.
| Tested Reactivity | Mouse |
| Host / Isotype | Hamster / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | H-2Kb-specific murine cytotoxic T-lymphocyte (CTL) clone BM10-37 相同性解析による交差性が予測される生物種 |
| Full Name | CD3 antigen, epsilon polypeptide |
| GenBank accession number | BC098236 |
| Gene symbol | CD3ε |
| Gene ID (NCBI) | 12501 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.09% sodium azide. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 CD3ε antibody CL488-65060 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
