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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 647 Anti-Mouse CD3ε (145-2C11)

CD3ε Monoclonal Antibody for FC

Host / Isotype

Hamster / IgG

Reactivity

mouse

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

145-2C11

Cat no : CL647-65060

Synonyms

Cd3e, T3e, CD3epsilon, CD3, AI504783

Validation Data Gallery

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  • Flow cytometry (FC) experiment of mouse splenocytes using CoraLite® Plus 647 Anti-Mouse CD3ε (145-2C11) (CL647-65060)

    FC experiment of mouse splenocytes using CL647-65060

    1x10^6 mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse CD3ε (145-2C11) (CL647-65060, Clone:145-2C11) or 0.25 ug Isotype Control, and 0.25 ug PE Anti-Mouse CD4 (GK1.5) (PE-65104, Clone: GK1.5). Cells were not fixed.

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Tested Applications

Positive FC detected inmouse splenocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65060 targets CD3ε in FC applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Hamster / IgG
Class Monoclonal
Type Antibody
Immunogen H-2Kb-specific murine cytotoxic T-lymphocyte (CTL) clone BM10-37 相同性解析による交差性が予測される生物種
Full Name CD3 antigen, epsilon polypeptide
GenBank accession numberBC098236
Gene symbol CD3ε
Gene ID (NCBI) 12501
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 CD3ε antibody CL647-65060Download protocol
Standard Protocols
Click here to view our Standard Protocols