CoraLite®594 Anti-Mouse CD31 (390)
CD31 Monoclonal Antibody for IF/ICC, FC
Host / Isotype
Rat / IgG2a, kappa
Reactivity
mouse
Applications
IF/ICC, FC
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
390
Cat no : CL594-65058
Synonyms
Validation Data Gallery
Tested Applications
Positive IF/ICC detected in | bEnd.3 cells |
Positive FC detected in | mouse bone marrow cells |
Recommended dilution
Application | Dilution |
---|---|
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL594-65058 targets CD31 in IF/ICC, FC applications and shows reactivity with mouse samples.
Tested Reactivity | mouse |
Host / Isotype | Rat / IgG2a, kappa |
Class | Monoclonal |
Type | Antibody |
Immunogen | C3H/HeJ mouse hematopoietic progenitor cell line 32D 相同性解析による交差性が予測される生物種 |
Full Name | platelet/endothelial cell adhesion molecule 1 |
GenBank accession number | BC008519 |
Gene symbol | CD31 |
Gene ID (NCBI) | 18613 |
RRID | AB_2919928 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Affinity purification |
Storage Buffer | PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3. |
Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).
Protocols
Product Specific Protocols | |
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IF protocol for CL594 CD31 antibody CL594-65058 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |