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Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD314/NKG2D (1D11) (65188-1-Ig)

    FC experiment of human PBMCs using 65188-1-Ig

    1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD314/NKG2D (65188-1-Ig, Clone:1D11) or Mouse IgG1 Isotype Control and CoraLite®647-conjugated F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8) at dilution 1:1000. Cells were then stained with CL488-conjugated CD8 (CL488-65135). Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD314/NKG2D (1D11) (65188-1-Ig)

    FC experiment of human PBMCs using 65188-1-Ig

    1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD314/NKG2D (65188-1-Ig, Clone:1D11) or Mouse IgG1 Isotype Control and CoraLite®647-conjugated F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

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Anti-Human CD314/NKG2D (1D11)

CD314/NKG2D Monoclonal Antibody for FC
Cat No. 65188-1-Ig
Clone No.1D11

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Synonyms

CD314, D12S2489E, FLJ17759, FLJ75772, KLR, KLRK1, NK cell receptor D, NKG2 D, NKG2 D activating NK receptor, NKG2D

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65188-1-Ig targets CD314/NKG2D in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen NKL cell line 相同性解析による交差性が予測される生物種
Full Name killer cell lectin-like receptor subfamily K, member 1
Calculated molecular weight 25 kDa
GenBank accession numberBC039836
Gene Symbol KLRK1
Gene ID (NCBI) 22914
RRIDAB_2918459
Conjugate Unconjugated
Form Liquid
Purification MethodPurified by protein-A affinity chromatography
UNIPROT IDP26718
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD314, also known as NKG2D or Killer cell lectin-like receptor subfamily K member 1 (KLRK1), is a type II lectin-like transmembrane stimulatory receptor (PMID: 8436421). In humans, it is expressed on NK cells, gamma delta T cells, and CD8+ alpha beta T cells (PMID: 10426993). Various families of cell surface ligands have been identified, including the MICA/MICB and ULBP proteins (PMID: 12150888). CD314 is involved in both innate and adaptive immunities, and the NKG2D/NKG2DL pathway involves multiple effector cell types for controlling tumor progression (PMID: 31720075).

Protocols

Product Specific Protocols
FC protocol for CD314/NKG2D antibody 65188-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols