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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PE Anti-Human CD314/NKG2D (1D11)

CD314/NKG2D Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

1D11

Cat no : PE-65188

Synonyms

CD314, D12S2489E, FLJ17759, FLJ75772, KLR, KLRK1, NK cell receptor D, NKG2 D, NKG2 D activating NK receptor, NKG2D

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human peripheral blood lymphocytes using PE Anti-Human CD314/NKG2D (1D11) (PE-65188)

    FC experiment of human peripheral blood lymphocytes using PE-65188

    1X10^6 human peripheral blood lymphocytes were surface stained with APC Anti-Human CD8 (APC-65146, Clone: SK1) and 10 ul PE Anti-Human CD314/NKG2D (PE-65188, Clone: 1D11). Cells were not fixed.

  • Flow cytometry (FC) experiment of human peripheral blood lymphocytes using PE Anti-Human CD314 (1D11) (PE-65188)

    FC experiment of human peripheral blood lymphocytes using PE-65188

    1X10^6 human peripheral blood lymphocytes were surface stained with APC Anti-Human CD8 (APC-65146, Clone: SK1) and PE-Mouse IgG1 isotype control. Cells were not fixed.

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Tested Applications

Positive FC detected inhuman peripheral blood lymphocytes

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 µl per 10^6 cells in a 100 µl suspension or 10 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

PE-65188 targets CD314/NKG2D in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen NKL cell line 相同性解析による交差性が予測される生物種
Full Name killer cell lectin-like receptor subfamily K, member 1
Calculated molecular weight 25 kDa
GenBank accession numberBC039836
Gene symbol KLRK1
Gene ID (NCBI) 22914
RRIDAB_2883958
Conjugate PE Fluorescent Dye
Excitation/Emission maxima wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification MethodThe purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Storage Buffer PBS with 0.1% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD314, also known as NKG2D or Killer cell lectin-like receptor subfamily K member 1 (KLRK1), is a type II lectin-like transmembrane stimulatory receptor (PMID: 8436421). In humans, it is expressed on NK cells, gamma delta T cells, and CD8+ alpha beta T cells (PMID: 10426993). Various families of cell surface ligands have been identified, including the MICA/MICB and ULBP proteins (PMID: 12150888). CD314 is involved in both innate and adaptive immunities, and the NKG2D/NKG2DL pathway involves multiple effector cell types for controlling tumor progression (PMID: 31720075).

Protocols

Product Specific Protocols
FC protocol for PE CD314/NKG2D antibody PE-65188Download protocol
Standard Protocols
Click here to view our Standard Protocols