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Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using CoraLite® Plus 594 Anti-Human CD314/NKG2D (1D11) (CL594-65188)

    FC experiment of human PBMCs using CL594-65188

    1x10^6 human PBMCs were surface stained with CL750 Anti-Human CD8 and 5 ul CoraLite® Plus 594 Anti-Human CD314/NKG2D (CL594-65188, Clone:1D11) or CoraLite® Plus 594 Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using CoraLite® Plus 594 Anti-Human CD314/NKG2D (1D11) (CL594-65188)

    FC experiment of human PBMCs using CL594-65188

    1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 594 Anti-Human CD314/NKG2D (CL594-65188, Clone:1D11) or CoraLite® Plus 594 Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

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CoraLite® Plus 594 Anti-Human CD314/NKG2D (1D11)

CD314/NKG2D Monoclonal Antibody for FC
Cat No. CL594-65188
Clone No.1D11

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Synonyms

CD314, D12S2489E, FLJ17759, FLJ75772, KLR, KLRK1, NK cell receptor D, NKG2 D, NKG2 D activating NK receptor, NKG2D

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 594
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-65188 targets CD314/NKG2D in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen NKL cell line 相同性解析による交差性が予測される生物種
Full Name killer cell lectin-like receptor subfamily K, member 1
Calculated molecular weight 25 kDa
GenBank accession numberBC039836
Gene Symbol KLRK1
Gene ID (NCBI) 22914
RRIDAB_3673459
Conjugate CoraLite® Plus 594 Fluorescent Dye
Excitation/Emission maxima wavelengths594 nm / 615 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP26718
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD314, also known as NKG2D or Killer cell lectin-like receptor subfamily K member 1 (KLRK1), is a type II lectin-like transmembrane stimulatory receptor (PMID: 8436421). In humans, it is expressed on NK cells, gamma delta T cells, and CD8+ alpha beta T cells (PMID: 10426993). Various families of cell surface ligands have been identified, including the MICA/MICB and ULBP proteins (PMID: 12150888). CD314 is involved in both innate and adaptive immunities, and the NKG2D/NKG2DL pathway involves multiple effector cell types for controlling tumor progression (PMID: 31720075).

Protocols

Product Specific Protocols
FC protocol for CL Plus 594 CD314/NKG2D antibody CL594-65188Download protocol
Standard Protocols
Click here to view our Standard Protocols