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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 405 Anti-Human CD41a (HIP8)

CD41a Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human, non-human primates

Applications

FC

Conjugate

CoraLite® Plus 405 Fluorescent Dye

CloneNo.

HIP8

Cat no : CL405-65173

Synonyms

GTA, GPIIb, GPalpha IIb, GP2B, CD41B

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human peripheral blood platelets using CoraLite® Plus 405 Anti-Human CD41a (HIP8) (CL405-65173)

    FC experiment of human peripheral blood platelets using CL405-65173

    100 ul human platelet-rich plasma (diluted 1:2 with PBS) was surface stained with 5 ul CoraLite® Plus 405 Anti-Human CD41a (CL405-65173, Clone:HIP8) or CoraLite® Plus 405 Mouse IgG1 Isotype Control (CL405-65124). Cells were not fixed.

  • Flow cytometry (FC) experiment of human peripheral blood platelets using CoraLite® Plus 405 Anti-Human CD41a (HIP8) (CL405-65173)

    FC experiment of human peripheral blood platelets using CL405-65173

    100 ul human platelet-rich plasma (diluted 1:2 with PBS) was surface stained with 5 ul CoraLite® Plus 405 Anti-Human CD41a (CL405-65173, Clone:HIP8) or CoraLite® Plus 405 Mouse IgG1 Isotype Control (CL405-65124). Cells were not fixed.

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Tested Applications

Positive FC detected inhuman peripheral blood platelets

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is N/A per 10^6 cells in a 100 µl suspension or N/A per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL405-65173 targets CD41a in FC applications and shows reactivity with human, non-human primates samples.

Tested Reactivity human, non-human primates
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen N/A 相同性解析による交差性が予測される生物種
Full Name integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigen CD41)
GenBank accession numberBC117443
Gene symbol CD41a
Gene ID (NCBI) 3674
Conjugate CoraLite® Plus 405 Fluorescent Dye
Excitation/Emission maxima wavelengths399 nm / 422 nm
Form Liquid
Purification MethodUnknow
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

The integrins are a superfamily of cell adhesion receptors that bind to extracellular matrix ligands, cell-surface ligands, and soluble ligands (PMID: 17543136). They are transmembrane αβ heterodimers and at least 24 distinct integrin heterodimers are formed by the combination of 18 α and eight β known subunits (PMID: 17543136; 20029421). In addition to mediating cell adhesion, integrins also play important roles in modulating signal transduction pathways that control cellular responses including migration, proliferation, differentiation, and apoptosis (PMID:19118207). Integrin alpha-IIb (ITGA2B, CD41) is expressed on platelets, megakaryocytes and some hematopoietic progenitor cells (PMID: 11934866).

Protocols

Product Specific Protocols
FC protocol for CL Plus 405 CD41a antibody CL405-65173Download protocol
Standard Protocols
Click here to view our Standard Protocols