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Validation Data Gallery

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  • Flow cytometry (FC) experiment of human peripheral blood platelets using CoraLite® Plus 647 Anti-Human CD42b (AK2) (CL647-65163)

    FC experiment of human peripheral blood platelets using CL647-65163

    1X10^6 human peripheral blood platelets were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD42b (CL647-65163, Clone: AK2) or unstained. Cells were not fixed.

  • Flow cytometry (FC) experiment of human peripheral blood platelets using CoraLite® Plus 647 Anti-Human CD42b (AK2) (CL647-65163)

    FC experiment of human peripheral blood platelets using CL647-65163

    1X10^6 human peripheral blood platelets were surface co-stained with FITC Anti-Human CD41a and 5 ul CoraLite® Plus 647 Anti-Human CD42b (CL647-65163, Clone: AK2) or unstained. Cells were not fixed.

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CoraLite® Plus 647 Anti-Human CD42b (AK2)

CD42b Monoclonal Antibody for FC
Cat No. CL647-65163
Clone No.AK2

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC

Synonyms

BSS, CD42B, CD42b alpha, GP1B, GP1BA

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 647
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC detected inhuman peripheral blood platelets

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65163 targets CD42b in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Human platelets 相同性解析による交差性が予測される生物種
Full Name glycoprotein Ib (platelet), alpha polypeptide
Calculated molecular weight 626 aa, 69 kDa
GenBank accession numberBC027955
Gene Symbol CD42b
Gene ID (NCBI) 2811
ENSEMBL Gene IDENSG00000185245
RRIDAB_2934993
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodPurified by protein-A affinity chromatography
UNIPROT IDP07359
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD42b, also known as platelet glycoprotein Ib alpha chain (GPIb alpha), is a type I transmembrane glycoprotein of 135-145 kDa (PMID: 2656709). It is expressed on platelets and megakaryocytes. CD42b and CD42c (GPIb beta) are linked by a disulphide bond to form GPIb (PMID: 7660135). GPIb forms a noncovalent complex with CD42a (GPIX) and CD42d (GPV) and acts as a receptor for von Willebrand factor (vWF) and thrombin (PMID: 7660135; 3759960). The GPIb-V-IX complex mediates vWF-dependent platelet adhesion to blood vessels. Defects in the expression of CD42b result in Bernard-Soulier syndromes and platelet-type von Willebrand disease (PMID: 9371310).

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 CD42b antibody CL647-65163Download protocol
Standard Protocols
Click here to view our Standard Protocols