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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated CD44 Monoclonal antibody

CD44 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

6F4H2

Cat no : CL488-60224

Synonyms

ECMR-III, ECMR III, CSPG8, CDW44, CD44 antigen

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CoraLite® Plus 488-conjugated CD44 Monoclonal anti (CL488-60224)

    IF Staining of A431 using CL488-60224

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using CoraLite® Plus 488 CD44 antibody (CL488-60224, Clone: 6F4H2 ) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-60224 targets CD44 in IF/ICC applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CD44 fusion protein Ag7633 相同性解析による交差性が予測される生物種
Full Name CD44 molecule (Indian blood group)
Calculated molecular weight 742 aa, 82 kDa
Observed molecular weight 80-95 kDa
GenBank accession numberBC004372
Gene symbol CD44
Gene ID (NCBI) 960
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD44 is a type I transmembrane glycoprotein that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands. Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. This protein exists in multiple forms (with molecular weight ranging from 16 kDa to over 200 kDa) generated by alternative RNA splicing and extensive post-translational modifications.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CD44 antibody CL488-60224Download protocol
Standard Protocols
Click here to view our Standard Protocols