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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human lung cancer tissue using CoraLite®594-conjugated CD44 Monoclonal antibody (CL594-60224)

    IF Staining of human lung cancer using CL594-60224

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite®594 CD44 antibody (CL594-60224, Clone: 6F4H2 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of human lung cancer tissue using CoraLite®594-conjugated CD44 Monoclonal antibody (CL594-60224)

    IF Staining of human lung cancer using CL594-60224

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite®594 CD44 antibody (CL594-60224, Clone: 6F4H2 ) at dilution of 1:200.

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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated CD44 Monoclonal antibody

CD44 Monoclonal Antibody for IF-P
Cat No. CL594-60224
Clone No.6F4H2

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig

Applications

IF-P

Synonyms

ECMR-III, ECMR III, CSPG8, CDW44, CD44 antigen

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF-P detected inhuman lung cancer tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-60224 targets CD44 in IF-P applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CD44 fusion protein Ag7633 相同性解析による交差性が予測される生物種
Full Name CD44 molecule (Indian blood group)
Calculated molecular weight 742 aa, 82 kDa
GenBank accession numberBC004372
Gene Symbol CD44
Gene ID (NCBI) 960
ENSEMBL Gene IDENSG00000026508
RRIDAB_2883445
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP16070
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD44 is a type I transmembrane glycoprotein that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands. Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. This protein exists in multiple forms (with molecular weight ranging from 16 kDa to over 200 kDa) generated by alternative RNA splicing and extensive post-translational modifications. The antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.

Protocols

Product Specific Protocols
IF protocol for CL594 CD44 antibody CL594-60224Download protocol
Standard Protocols
Click here to view our Standard Protocols