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Validation Data Gallery

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  • Flow cytometry (FC) experiment of human peripheral blood leukocyte using Biotin Anti-Human CD45 Rabbit Recombinant Antibody (Biotin-98117)

    FC experiment of human peripheral blood leukocyte using Biotin-98117

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Biotin Anti-Human CD45 Rabbit RecAb (Biotin-98117, Clone: 241670E10) and CoraLite®488-conjugated streptavidin, or unstained. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Flow cytometry (FC) experiment of human peripheral blood leukocyte using Biotin Anti-Human CD45 Rabbit Recombinant Antibody (Biotin-98117)

    FC experiment of human peripheral blood leukocyte using Biotin-98117

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Biotin Anti-Human CD45 Rabbit RecAb (Biotin-98117, Clone: 241670E10) and CoraLite®488-conjugated streptavidin (red), or unstained (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Biotin Anti-Human CD45 Rabbit Recombinant Antibody

CD45 Uni-rAbTM Recombinant Antibody for FC
Cat No. Biotin-98117
Clone No.241670E10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

LCA, L CA, GP180, EC:3.1.3.48, B220

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Biotin
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC detected inhuman peripheral blood leukocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-98117 targets CD45 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, receptor type, C
Gene Symbol CD45
Gene ID (NCBI) 5788
ENSEMBL Gene IDENSG00000081237
Conjugate Biotin
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide , pH 7.3
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).

Protocols

Product Specific Protocols
FC protocol for Biotin CD45 antibody Biotin-98117Download protocol
Standard Protocols
Click here to view our Standard Protocols