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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 647 Anti-Human CD45 Rabbit Recombinant Antibody

CD45 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

241670E10

Cat no : CL647-98117

Synonyms

L CA, GP180, EC:3.1.3.48, CD45R, B220

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human peripheral blood leukocytes using CoraLite® Plus 647 Anti-Human CD45 Rabbit Recombin (CL647-98117)

    FC experiment of human peripheral blood leukocytes using CL647-98117

    1x10^6 human peripheral blood leukocytes were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD45 Rabbit Recombinant Antibody (CL647-98117, Clone: 241670E10) or CoraLite® Plus 647 Rabbit IgG Isotype Control Recombinant Antibody (CL647-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Flow cytometry (FC) experiment of human peripheral blood leukocytes using CoraLite® Plus 647 Anti-Human CD45 Rabbit Recombin (CL647-98117)

    FC experiment of human peripheral blood leukocytes using CL647-98117

    1x10^6 human peripheral blood leukocytes were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD45 Rabbit Recombinant Antibody (CL647-98117, Clone: 241670E10) (red) or CoraLite® Plus 647 Rabbit IgG Isotype Control Recombinant Antibody (CL647-98136, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Tested Applications

Positive FC detected inhuman peripheral blood leukocytes

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL647-98117 is CD45 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, receptor type, C
Gene symbol CD45
Gene ID (NCBI) 5788
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 CD45 antibody CL647-98117Download protocol
Standard Protocols
Click here to view our Standard Protocols