CoraLite® Plus 488 Anti-Mouse CD69 (H1.2F3)
CD69 Monoclonal Antibody for FC
Host / Isotype
Armenian Hamster / IgG
Reactivity
Mouse
Applications
FC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
H1.2F3
Cat no : CL488-65105
Synonyms
Validation Data Gallery
Tested Applications
| Positive FC detected in | Anti-CD3/CD28 treated mouse splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-65105 targets CD69 in FC applications and shows reactivity with Mouse samples.
| Tested Reactivity | Mouse |
| Host / Isotype | Armenian Hamster / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Mouse dendritic epidermal T cell line Y245 相同性解析による交差性が予測される生物種 |
| Full Name | CD69 antigen |
| GenBank accession number | BC106997 |
| Gene symbol | Cd69 |
| Gene ID (NCBI) | 12515 |
| RRID | AB_2883179 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.09% sodium azide. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD69, also known as AIM, EA-1, Leu-23, and MLR3, is a type II transmembrane glycoprotein that belongs to the C-type lectin superfamily (PMID: 8340758; 7804122). CD69 is constitutively expressed by mature thymocytes, platelets, several subsets of tissue resident immune cells (including resident memory T cells and gamma delta T cells), and is inducibly expressed by activated T cells, B cells, natural killer (NK) cells, monocytes, neutrophils (PMID: 8100776; 28475283). CD69 has been identified as an early activation marker of lymphocytes and is commonly used as a marker of activated lymphocytes and NK cells (PMID: 28475283; 25759842). It is involved in the regulation of immune responses (PMID: 15745855).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 CD69 antibody CL488-65105 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
