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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 555 Anti-Mouse CD69 (H1.2F3)

CD69 Monoclonal Antibody for FC

Host / Isotype

Armenian Hamster / IgG

Reactivity

Mouse

Applications

FC

Conjugate

CoraLite® Plus 555 Fluorescent Dye

CloneNo.

H1.2F3

Cat no : CL555-65105

Synonyms

5830438K24Rik, AI452015, AIM, Cd69, CD69 antigen, VEA

Validation Data Gallery

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  • Flow cytometry (FC) experiment of mouse splenocytes using CoraLite® Plus 555 Anti-Mouse CD69 (H1.2F3) (CL555-65105)

    FC experiment of mouse splenocytes using CL555-65105

    1X10^6 unstimulated or anti-CD3/CD28-stimulated mouse splenocytes were surface co-stained with CoraLite® Plus 647 Anti-Mouse CD3 (17A2) and 0.5 ug CoraLite® Plus 555 Anti-Mouse CD69 (CL555-65105, Clone:H1.2F3) or 0.5 ug Isotype Control. Cells were not fixed.

  • Flow cytometry (FC) experiment of mouse splenocytes using CoraLite® Plus 555 Anti-Mouse CD69 (H1.2F3) (CL555-65105)

    FC experiment of mouse splenocytes using CL555-65105

    1X10^6 anti-CD3/CD28-stimulated mouse splenocytes were surface stained with 0.5 ug CoraLite® Plus 555 Anti-Mouse CD69 (CL555-65105, Clone:H1.2F3) (red), or 0.5 ug Isotype Control (blue). The black dashed line indicates unstimulated mouse splenocytes surface stained with 0.5 ug CoraLite® Plus 555 Anti-Mouse CD69 (CL555-65105, Clone:H1.2F3). Cells were not fixed.

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Tested Applications

Positive FC detected inAnti-CD3/CD28-stimulated mouse splenocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-65105 targets CD69 in FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Armenian Hamster / IgG
Class Monoclonal
Type Antibody
Immunogen Mouse dendritic epidermal T cell line Y245 相同性解析による交差性が予測される生物種
Full Name CD69 antigen
GenBank accession numberBC106997
Gene symbol Cd69
Gene ID (NCBI) 12515
Conjugate CoraLite® Plus 555 Fluorescent Dye
Excitation/Emission maxima wavelengths554 nm / 570 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD69, also known as AIM, EA-1, Leu-23, and MLR3, is a type II transmembrane glycoprotein that belongs to the C-type lectin superfamily (PMID: 8340758; 7804122). CD69 is constitutively expressed by mature thymocytes, platelets, several subsets of tissue resident immune cells (including resident memory T cells and gamma delta T cells), and is inducibly expressed by activated T cells, B cells, natural killer (NK) cells, monocytes, neutrophils (PMID: 8100776; 28475283). CD69 has been identified as an early activation marker of lymphocytes and is commonly used as a marker of activated lymphocytes and NK cells (PMID: 28475283; 25759842). It is involved in the regulation of immune responses (PMID: 15745855).

Protocols

Product Specific Protocols
FC protocol for CL Plus 555 CD69 antibody CL555-65105Download protocol
Standard Protocols
Click here to view our Standard Protocols