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CoraLite® Plus 488-conjugated CD71 Monoclonal antibody
CD71 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG2a
Reactivity
human
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
3C11F11
Cat no : CL488-66180
Synonyms
Validation Data Gallery
Tested Applications
Positive IF/ICC detected in | K-562 cells, MCF-7 cells |
Recommended dilution
Application | Dilution |
---|---|
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL488-66180 targets CD71 in IF/ICC applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | CD71 fusion protein Ag21612 相同性解析による交差性が予測される生物種 |
Full Name | transferrin receptor (p90, CD71) |
Calculated molecular weight | 85 kDa |
Observed molecular weight | 90 kDa |
GenBank accession number | BC001188 |
Gene symbol | CD71 |
Gene ID (NCBI) | 7037 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex which enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 488 CD71 antibody CL488-66180 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |