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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated CD71 Monoclonal antibody

CD71 Monoclonal Antibody for IF/ICC, IF-P

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC, IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3C11F11

Cat no : CL594-66180

Synonyms

CD71, p90, sTfR, T9, TFR, TFR1, TFRC, TR, Transferrin receptor protein 1, TRFR

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using CoraLite®594-conjugated CD71 Monoclonal antibody (CL594-66180)

    IF Staining of human breast cancer using CL594-66180

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®594 CD71 antibody (CL594-66180, Clone: 3C11F11 ) at dilution of 1:100.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using CoraLite®594-conjugated CD71 Monoclonal antibody (CL594-66180)

    IF Staining of human breast cancer using CL594-66180

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®594 CD71 antibody (CL594-66180, Clone: 3C11F11 ) at dilution of 1:100.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CoraLite®594-conjugated CD71 Monoclonal antibody (CL594-66180)

    IF Staining of MCF-7 using CL594-66180

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite®594 CD71 antibody (CL594-66180, Clone: 3C11F11 ) at dilution of 1:200.

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Tested Applications

Positive IF-P detected inhuman breast cancer tissue
Positive IF/ICC detected inMCF-7 cells, human breast cancer tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66180 targets CD71 in IF/ICC, IF-P applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CD71 fusion protein Ag21612 相同性解析による交差性が予測される生物種
Full Name transferrin receptor (p90, CD71)
Calculated molecular weight 85 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC001188
Gene symbol CD71
Gene ID (NCBI) 7037
RRIDAB_2919961
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex which enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.

Protocols

Product Specific Protocols
IF protocol for CL594 CD71 antibody CL594-66180Download protocol
Standard Protocols
Click here to view our Standard Protocols