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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 594 Anti-Human CD71 (OKT9)

CD71 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Conjugate

CoraLite® Plus 594 Fluorescent Dye

CloneNo.

OKT9

Cat no : CL594-65235

Synonyms

CD71, p90, sTfR, T9, TFR, TFR1, TFRC, TR, Transferrin receptor protein 1, TRFR

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using CoraLite® Plus 594 Anti-Human CD71 (OKT9) (CL594-65235)

    FC experiment of human PBMCs using CL594-65235

    1x10^6 PHA treated (3 days) human PBMCs were surface stained with 5 ul CoraLite® Plus 594 Anti-Human CD71 (CL594-65235, Clone:OKT9) (red) or CoraLite®594 Mouse IgG1 Isotype Control (blue). 1x10^6 untreated human PBMCs were surface stained with 5 ul CoraLite® Plus 594 Anti-Human CD71 (CL594-65235, Clone:OKT9) (black dashed). Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using CoraLite® Plus 594 Anti-Human CD71 (OKT9) (CL594-65235)

    FC experiment of human PBMCs using CL594-65235

    1x10^6 PHA treated (3 days) human PBMCs were surface stained with CL750 Anti-Human CD3 and 5 ul CoraLite® Plus 594 Anti-Human CD71 (CL594-65235, Clone:OKT9) (right) or CoraLite®594 Mouse IgG1 Isotype Control (left). 1x10^6 untreated human PBMCs were surface stained with 5 ul CoraLite® Plus 594 Anti-Human CD71 (CL594-65235, Clone:OKT9) (center). Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Lymphocytes were gated.

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Tested Applications

Positive FC detected inPHA treated human PBMCs

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-65235 targets CD71 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen Human T-cell acute lymphoblastic leukemia cells 相同性解析による交差性が予測される生物種
Full Name transferrin receptor (p90, CD71)
Calculated molecular weight 85 kDa
GenBank accession numberBC001188
Gene symbol CD71
Gene ID (NCBI) 7037
Conjugate CoraLite® Plus 594 Fluorescent Dye
Excitation/Emission maxima wavelengths594 nm / 615 nm
Form Liquid
Purification MethodN/A
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex that enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.

Protocols

Product Specific Protocols
FC protocol for CL Plus 594 CD71 antibody CL594-65235Download protocol
Standard Protocols
Click here to view our Standard Protocols