CoraLite® Plus 488-conjugated CD98/SLC3A2 Monoclonal antibody
CD98/SLC3A2 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG2a
Reactivity
human
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2B10F5
Cat no : CL488-66883
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-66883 targets CD98/SLC3A2 in IF/ICC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CD98/SLC3A2 fusion protein Ag7301 相同性解析による交差性が予測される生物種 |
| Full Name | solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2 |
| Calculated molecular weight | 68 kDa |
| Observed molecular weight | 85-94 kDa, 120-130 kDa |
| GenBank accession number | BC001061 |
| Gene symbol | CD98 |
| Gene ID (NCBI) | 6520 |
| RRID | AB_2919385 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CD98 is a cell-surface heterodimer consisting of a heavy chain (CD98hc) and a light chain. CD98hc also interacts with certain integrin b-subunits to regulate cell migration, survival, proliferation, and adhesion/polarity. CD98hc is overexpressed on the cell surface of many cancers and increased CD98hc expression is associated with the development and progression of tumors.(PMID:25084765). This protein has 4 isoforms with the molecular mass of 58-71 kDa and can be detected 85-94 kDa due to glycosylation. The glycosylated CD98hc can links to a non-glycosylated light chain (~40 kDa) via a disulfide bond to form a heterodimeric CD98 antigen with molecular mass of 120-130 kDa (PMID: 14770309).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 CD98/SLC3A2 antibody CL488-66883 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
