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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated CD98/SLC3A2 Monoclonal antibody

CD98/SLC3A2 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2B10F5

Cat no : CL594-66883

Synonyms

CD98, SLC3A2, MDU1, Amino acid transporter heavy chain SLC3A2, 4F2 cell-surface antigen heavy chain

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated CD98/SLC3A2 Monoclonal ant (CL594-66883)

    IF Staining of HeLa using CL594-66883

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 CD98/SLC3A2 antibody (CL594-66883, Clone: 2B10F5 ) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66883 targets CD98/SLC3A2 in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CD98/SLC3A2 fusion protein Ag7301 相同性解析による交差性が予測される生物種
Full Name solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2
Calculated molecular weight 68 kDa
Observed molecular weight 85-94 kDa, 120-130 kDa
GenBank accession numberBC001061
Gene symbol CD98
Gene ID (NCBI) 6520
RRIDAB_2920052
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD98 is a cell-surface heterodimer consisting of a heavy chain (CD98hc) and a light chain. CD98hc also interacts with certain integrin b-subunits to regulate cell migration, survival, proliferation, and adhesion/polarity. CD98hc is overexpressed on the cell surface of many cancers and increased CD98hc expression is associated with the development and progression of tumors.(PMID:25084765). This protein has 4 isoforms with the molecular mass of 58-71 kDa and can be detected 85-94 kDa due to glycosylation. The glycosylated CD98hc can links to a non-glycosylated light chain (~40 kDa) via a disulfide bond to form a heterodimeric CD98 antigen with molecular mass of 120-130 kDa (PMID: 14770309).

Protocols

Product Specific Protocols
IF protocol for CL594 CD98/SLC3A2 antibody CL594-66883Download protocol
Standard Protocols
Click here to view our Standard Protocols