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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated CLTA Polyclonal anti (CL488-10852)

    IF Staining of HepG2 using CL488-10852

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 CLTA antibody (CL488-10852) at dilution of 1:200, CL594-phalloidin (red).

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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated CLTA Polyclonal antibody

CLTA Polyclonal Antibody for IF/ICC
Cat No. CL488-10852

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

LCA, clathrin, light chain (Lca), Clathrin light chain A

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-10852 targets CLTA in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CLTA fusion protein Ag1299 相同性解析による交差性が予測される生物種
Full Name clathrin, light chain (Lca)
Calculated molecular weight 27 kDa
Observed molecular weight 35-38 kDa
GenBank accession numberBC019287
Gene Symbol CLTA
Gene ID (NCBI) 1211
RRIDAB_2919004
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP09496
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. The clathrin molecule has a triskelion shape. Each clathrin triskelion is composed of three identical heavy chains (180 kDa) and three light chains of two types, LCA (CLTA) and LCB (CLTB) (30-40 kDa). The light chain subunits are thought to regulate the formation or disassembly of clathrin coats. (PMID: 2445759; 8374173; 3563513)

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CLTA antibody CL488-10852Download protocol
Standard Protocols
Click here to view our Standard Protocols