Validation Data Gallery
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Tested Applications
| Positive WB detected in | A549 cells, pig brain tissue, rat brain tissue, NCI-H1299 cells, JAR cells, Jurkat cells, K-562 cells |
| Positive IF/ICC detected in | hTERT-RPE1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:175-1:700 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67061-1-Ig targets CNTROB in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.
| Tested Reactivity | Human, Mouse, Rat, Pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CNTROB fusion protein Ag25447 相同性解析による交差性が予測される生物種 |
| Full Name | centrobin, centrosomal BRCA2 interacting protein |
| Calculated molecular weight | 903 aa, 101 kDa |
| Observed molecular weight | 97 kDa |
| GenBank accession number | BC021134 |
| Gene Symbol | CNTROB |
| Gene ID (NCBI) | 116840 |
| RRID | AB_2882371 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q8N137 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CNTROB antibody 67061-1-Ig | Download protocol |
| IF protocol for CNTROB antibody 67061-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |