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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CNTROB Monoclonal antibody, PBS Only (Detector)

CNTROB Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3D12A4

Cat no : 67061-1-PBS

Synonyms

LYST-interacting protein 8, LYST interacting protein 8, LIP8, Centrosomal BRCA2-interacting protein, Centrobin

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CNTROB Monoclonal antibody (67061-1-Ig)

    WB analysis using 67061-1-Ig (same clone as 67061-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67061-1-Ig (CNTROB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67061-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using CNTROB Monoclonal antibody (67061-1-Ig)

    WB analysis of pig brain using 67061-1-Ig (same clone as 67061-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 67061-1-Ig (CNTROB antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67061-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using CNTROB Monoclonal antibody (67061-1-Ig)

    WB analysis of rat brain using 67061-1-Ig (same clone as 67061-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 67061-1-Ig (CNTROB antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67061-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using CNTROB Monoclonal antibody (67061-1-Ig)

    IF Staining of hTERT-RPE1 using 67061-1-Ig (same clone as 67061-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using CNTROB antibody (67061-1-Ig, Clone: 3D12A4 ) at dilution of 1:350 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), ARL13B antibody (17711-1-AP, red). This data was developed using the same antibody clone with 67061-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50605-2

    Cytometric bead array standard curve of MP50605-2

    Cytometric bead array standard curve of MP50605-2, CNTROB Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67061-4-PBS. Detection antibody: 67061-1-PBS. Standard:Ag25447. Range: 1.563-100 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67061-1-PBS targets CNTROB as part of a matched antibody pair:

MP50605-2: 67061-4-PBS capture and 67061-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CNTROB fusion protein Ag25447 相同性解析による交差性が予測される生物種
Full Name centrobin, centrosomal BRCA2 interacting protein
Calculated molecular weight 903 aa, 101 kDa
Observed molecular weight 97 kDa
GenBank accession numberBC021134
Gene symbol CNTROB
Gene ID (NCBI) 116840
RRIDAB_2882371
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.