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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of C2C12 cells using CoraLite® Plus 488-conjugated Calponin Polyclonal (CL488-13938)

    IF Staining of C2C12 using CL488-13938

    Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using CoraLite® Plus 488 Calponin antibody (CL488-13938) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using CoraLite® Plus 488-conjugated Calponin Polyclonal (CL488-13938)

    IF Staining of MDCK using CL488-13938

    Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using CoraLite® Plus 488 Calponin antibody (CL488-13938) at dilution of 1:200.

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  • KD/KO Validated

CoraLite® Plus 488-conjugated Calponin Polyclonal antibody

Calponin Polyclonal Antibody for IF/ICC
Cat No. CL488-13938

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, dog

Applications

IF/ICC

Synonyms

CNN1, CNN 1, Calponin-1, Calponin 1, Basic calponin

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inC2C12 cells, MDCK cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-13938 targets Calponin in IF/ICC applications and shows reactivity with human, mouse, rat, dog samples.

Tested Reactivity human, mouse, rat, dog
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Calponin fusion protein Ag4989 相同性解析による交差性が予測される生物種
Full Name calponin 1, basic, smooth muscle
Calculated molecular weight 33 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC022015
Gene Symbol Calponin
Gene ID (NCBI) 1264
RRIDAB_2919081
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP51911
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Calponin is a family of actin filament-associated proteins which regulate smooth muscle cell contraction. Three isoforms of calponin exist: calponin h1 (CNN1) , calponin h2 (CNN2) and calponin 3 (CNN3). CNN1 is a basic 34-kD protein specifically expressed in smooth muscle and a marker of smooth muscle cell differentiation. This antibody raised against the full-length of human CNN1 may cross-react with CNN2 and CNN3.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Calponin antibody CL488-13938Download protocol
Standard Protocols
Click here to view our Standard Protocols