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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated Calponin Polyclonal antibody

Calponin Polyclonal Antibody for IF/ICC, IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IF-P

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-13938

Synonyms

CNN1, CNN 1, Calponin-1, Calponin 1, Basic calponin

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using CoraLite®594-conjugated Calponin Polyclonal antibo (CL594-13938)

    IF Staining of mouse heart using CL594-13938

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using CoraLite®594 Calponin antibody (CL594-13938) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of C2C12 cells using CoraLite®594-conjugated Calponin Polyclonal antibo (CL594-13938)

    IF Staining of C2C12 using CL594-13938

    Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using CoraLite®594 Calponin antibody (CL594-13938) at dilution of 1:200.

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Tested Applications

Positive IF-P detected inmouse heart tissue
Positive IF/ICC detected inC2C12 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-13938 targets Calponin in IF/ICC, IF-P applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Calponin fusion protein Ag4989 相同性解析による交差性が予測される生物種
Full Name calponin 1, basic, smooth muscle
Calculated molecular weight 33 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC022015
Gene symbol Calponin
Gene ID (NCBI) 1264
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Calponin is a family of actin filament-associated proteins which regulate smooth muscle cell contraction. Three isoforms of calponin exist: calponin h1 (CNN1) , calponin h2 (CNN2) and calponin 3 (CNN3). CNN1 is a basic 34-kD protein specifically expressed in smooth muscle and a marker of smooth muscle cell differentiation. This antibody raised against the full-length of human CNN1 may cross-react with CNN2 and CNN3.

Protocols

Product Specific Protocols
IF protocol for CL594 Calponin antibody CL594-13938Download protocol
Standard Protocols
Click here to view our Standard Protocols