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Calretinin Monoclonal antibody

Calretinin Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 66496-1-Ig
Clone No.2D7A9

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IF-P, ELISA

CALB2, calbindin 2, CAL2, CAB29, 2D7A9

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inpig brain tissue, mouse brain tissue, rat brain tissue, U2OS cells, rat cerebellum tissue, U-251 cells, mouse cerebellum tissue, rabbit brain tissue
Positive IHC detected inhuman appendicitis tissue, human brain tissue, human cerebellum tissue, human colon tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman appendicitis tissue
Positive IF/ICC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66496-1-Ig targets Calretinin in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Calretinin fusion protein Ag2924 相同性解析による交差性が予測される生物種
Full Name calbindin 2
Calculated molecular weight 29 kDa
Observed molecular weight 29 kDa
GenBank accession numberBC015484
Gene Symbol Calretinin
Gene ID (NCBI) 794
RRIDAB_2868480
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP22676
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Calbindin 2 (calretinin), is an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Calretinin is a useful marker for differentiating malignant mesothelioma from carcinomas.

Protocols

Product Specific Protocols
WB protocol for Calretinin antibody 66496-1-IgDownload protocol
IHC protocol for Calretinin antibody 66496-1-IgDownload protocol
IF protocol for Calretinin antibody 66496-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Cell

Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms of Neurological Disease in Mice.

Authors - Haibo Zhou
mouseWB,IF

Acta Neuropathol Commun

TMEM106B deficiency impairs cerebellar myelination and synaptic integrity with Purkinje cell loss.

Authors - Tuancheng Feng
mouseWB,IF

Neurobiol Dis

Sex specific correlation between GABAergic disruption in the dorsal hippocampus and flurothyl seizure susceptibility after neonatal hypoxic-ischemic brain injury.

Authors - Charles R Lechner
mouseIF

Neurobiol Dis

Cytohesin-2 mediates group I metabotropic glutamate receptor-dependent mechanical allodynia through the activation of ADP ribosylation factor 6 in the spinal cord.

Authors - Akiko Ito
humanWB

J Cancer

A Prognostic Model of Angiogenesis and Neutrophil Extracellular Traps Related Genes Manipulating Tumor Microenvironment in Colon Cancer

Authors - Dongsheng Zhang
mouseIF

Invest Ophthalmol Vis Sci

Topical Ripasudil Suppresses Retinal Ganglion Cell Death in a Mouse Model of Normal Tension Glaucoma.

Authors - Kei Akaiwa
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