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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®555-conjugated Calretinin Monoclonal antibody

Calretinin Monoclonal Antibody for IF/ICC, IF-P

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF/ICC, IF-P

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

2D7A9

Cat no : CL555-66496

Synonyms

CALB2, calbindin 2, CAL2, CAB29, 2D7A9

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human appendicitis tissue using CoraLite®555-conjugated Calretinin Monoclonal anti (CL555-66496)

    IF Staining of human appendicitis using CL555-66496

    Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using CoraLite®555 Calretinin antibody (CL555-66496, Clone: 2D7A9 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of human appendicitis tissue using CoraLite®555-conjugated Calretinin Monoclonal anti (CL555-66496)

    IF Staining of human appendicitis using CL555-66496

    Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using CoraLite®555 Calretinin antibody (CL555-66496, Clone: 2D7A9 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using CoraLite®555-conjugated Calretinin Monoclonal anti (CL555-66496)

    IF Staining of SH-SY5Y using CL555-66496

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CoraLite®555 Calretinin antibody (CL555-66496, Clone: 2D7A9 ) at dilution of 1:200.

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Tested Applications

Positive IF-P detected inhuman appendicitis tissue
Positive IF/ICC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-66496 targets Calretinin in IF/ICC, IF-P applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Calretinin fusion protein Ag2924 相同性解析による交差性が予測される生物種
Full Name calbindin 2
Calculated molecular weight 29 kDa
Observed molecular weight 29 kDa
GenBank accession numberBC015484
Gene symbol Calretinin
Gene ID (NCBI) 794
RRIDAB_2919687
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Calbindin 2 (calretinin), is an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Calretinin is a useful marker for differentiating malignant mesothelioma from carcinomas.

Protocols

Product Specific Protocols
IF protocol for CL555 Calretinin antibody CL555-66496Download protocol
Standard Protocols
Click here to view our Standard Protocols