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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Biotin-conjugated Calretinin Monoclonal antibody

Calretinin Monoclonal Antibody for IHC

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, pig, rat

Applications

IHC

Conjugate

Biotin

CloneNo.

2D7A9

Cat no : Biotin-66496

Synonyms

29 kDa calbindin, CAB29, CAL2, CALB2, calbindin 2, Calretinin, CR

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human brain tissue using Biotin-conjugated Calretinin Monoclonal antibody (Biotin-66496)

    IHC staining of human brain using Biotin-66496

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using Biotin-66496 (Calretinin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using Biotin-conjugated Calretinin Monoclonal antibody (Biotin-66496)

    IHC staining of human brain using Biotin-66496

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using Biotin-66496 (Calretinin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Biotin-conjugated Calretinin Monoclonal antibody (Biotin-66496)

    IHC staining of mouse brain using Biotin-66496

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using Biotin-66496 (Calretinin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Biotin-conjugated Calretinin Monoclonal antibody (Biotin-66496)

    IHC staining of mouse brain using Biotin-66496

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using Biotin-66496 (Calretinin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive IHC detected inhuman brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-66496 targets Calretinin in IHC applications and shows reactivity with Human, mouse, pig, rat samples.

Tested Reactivity Human, mouse, pig, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Calretinin fusion protein Ag2924 相同性解析による交差性が予測される生物種
Full Name calbindin 2
Calculated molecular weight 29 kDa
GenBank accession numberBC015484
Gene symbol Calretinin
Gene ID (NCBI) 794
RRIDAB_2883074
Conjugate Biotin
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Calbindin 2 (calretinin), is an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Calretinin is a useful marker for differentiating malignant mesothelioma from carcinomas.

Protocols

Product Specific Protocols
IHC protocol for Biotin Calretinin antibody Biotin-66496Download protocol
Standard Protocols
Click here to view our Standard Protocols