Cytokeratin 5 Polyclonal antibody

Name: Cytokeratin 5 Polyclonal antibody
Brand: Proteintech
SKU: 28506-1-AP

Cytokeratin 5 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 28506-1-AP

Synonyms

CK 5, CK-5, keratin 5, KRT5, Cytokeratin5

Validation Data Gallery

Filter:

AllWBIHCIF-PIF/ICC

WB analysis using 28506-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 28506-1-AP (Cytokeratin 5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC staining of human tonsillitis using 28506-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28506-1-AP (Cytokeratin 5 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 28506-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28506-1-AP (Cytokeratin 5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human brown disease using 28506-1-AP
Immunohistochemical analysis of paraffin-embedded human brown disease slide using 28506-1-AP (Cytokeratin 5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human brown disease using 28506-1-AP
Immunohistochemical analysis of paraffin-embedded human brown disease slide using 28506-1-AP (Cytokeratin 5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 28506-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28506-1-AP (Cytokeratin 5 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 28506-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28506-1-AP (Cytokeratin 5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 28506-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28506-1-AP (Cytokeratin 5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of human tonsillitis using 28506-1-AP
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using Cytokeratin 5 antibody (28506-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of human tonsillitis using 28506-1-AP
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using Cytokeratin 5 antibody (28506-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of A431 using 28506-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Cytokeratin 5 antibody (28506-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 28506-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A431 cells, mouse skin tissues, rat skin tissues
Positive IHC detected in	human tonsillitis tissue, human brown disease, human lung cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	human tonsillitis tissue
Positive IF/ICC detected in	A431 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunohistochemistry (IHC)	IHC : 1:1000-1:4000
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below
IF	See 5 publications below

Product Information

28506-1-AP targets Cytokeratin 5 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Cytokeratin 5 fusion protein Ag25436 相同性解析による交差性が予測される生物種
Full Name	keratin 5
Calculated molecular weight	590 aa, 62 kDa
Observed molecular weight	62 kDa
GenBank accession number	BC024292
Gene symbol	Cytokeratin 5
Gene ID (NCBI)	3852
RRID	AB_2881159
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 5 antibody 28506-1-AP	Download protocol
IHC protocol for Cytokeratin 5 antibody 28506-1-AP	Download protocol
IF protocol for Cytokeratin 5 antibody 28506-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	J Invest Dermatol Circulating Exosomal miR-493-3p Affects Melanocyte Survival and Function by Regulating Epidermal Dopamine Concentration in Segmental Vitiligo. Authors - Li Dong View Article
mouse	IF	FASEB J Sperm immotility is associated with epididymis metabolism disorder in mice under obstructive azoospermia Authors - Sheng Gao View Article
human	WB	J Clin Lab Anal Hsa_circRNA_0017620 regulated cell progression of non-small-cell lung cancer via miR-520a-5p/KRT5 axis. Authors - Shan Chen View Article
mouse	IF	J Oral Biosci Epigenetic modifier G9a is involved in regulation of mouse tongue development Authors - Hisashi Ideno View Article
human	IF	Cancer Lett The Single Cell Immunogenomic Landscape after Neoadjuvant Immunotherapy combined Chemotherapy in Esophageal Squamous Cell Carcinoma Authors - Zhe Yi Wang View Article
human	IF	Bioact Mater Biomimetic hydrogels with mesoscale collagen architecture for patient-derived tumor organoids culture Authors - Jiaxin Wang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cytokeratin 5 Polyclonal antibody

Cytokeratin 5 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Validation Data Gallery

WB analysis using 28506-1-AP

IHC staining of human tonsillitis using 28506-1-AP

IHC staining of human tonsillitis using 28506-1-AP

IHC staining of human brown disease using 28506-1-AP

IHC staining of human brown disease using 28506-1-AP

IHC staining of human lung cancer using 28506-1-AP

IHC staining of human lung cancer using 28506-1-AP

IHC staining of human lung cancer using 28506-1-AP

IF Staining of human tonsillitis using 28506-1-AP

IF Staining of human tonsillitis using 28506-1-AP

IF Staining of A431 using 28506-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Circulating Exosomal miR-493-3p Affects Melanocyte Survival and Function by Regulating Epidermal Dopamine Concentration in Segmental Vitiligo.

Sperm immotility is associated with epididymis metabolism disorder in mice under obstructive azoospermia

Hsa_circRNA_0017620 regulated cell progression of non-small-cell lung cancer via miR-520a-5p/KRT5 axis.

Epigenetic modifier G9a is involved in regulation of mouse tongue development

The Single Cell Immunogenomic Landscape after Neoadjuvant Immunotherapy combined Chemotherapy in Esophageal Squamous Cell Carcinoma

Biomimetic hydrogels with mesoscale collagen architecture for patient-derived tumor organoids culture