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Cytokeratin 5 Polyclonal antibody

Cytokeratin 5 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 28506-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, ELISA

CK 5, CK-5, keratin 5, KRT5, Cytokeratin5

Formulation:  PBS and Azide
PBS and Azide
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inA431 cells, mouse skin tissues, rat skin tissues
Positive IHC detected inhuman tonsillitis tissue, human brown disease, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28506-1-AP targets Cytokeratin 5 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cytokeratin 5 fusion protein Ag25436 相同性解析による交差性が予測される生物種
Full Name keratin 5
Calculated molecular weight 590 aa, 62 kDa
Observed molecular weight 62 kDa
GenBank accession numberBC024292
Gene Symbol Cytokeratin 5
Gene ID (NCBI) 3852
RRIDAB_2881159
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP13647
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cytokeratin 5 (CK5) is a type II intermediate filament protein predominantly expressed in basal epithelial cells of various tissues, including the skin, breast, salivary glands, urinary tract, and upper aerodigestive tract. It is commonly found in specialized cell types such as squamous cells, basal cells, and myoepithelial cells. CK5 forms heterodimers with cytokeratin 14 and plays a crucial role in providing structural support to epithelial cells

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 5 antibody 28506-1-APDownload protocol
IHC protocol for Cytokeratin 5 antibody 28506-1-APDownload protocol
IF protocol for Cytokeratin 5 antibody 28506-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

J Invest Dermatol

Circulating Exosomal miR-493-3p Affects Melanocyte Survival and Function by Regulating Epidermal Dopamine Concentration in Segmental Vitiligo.

Authors - Li Dong
mouseIF

FASEB J

Sperm immotility is associated with epididymis metabolism disorder in mice under obstructive azoospermia

Authors - Sheng Gao
humanWB

J Clin Lab Anal

Hsa_circRNA_0017620 regulated cell progression of non-small-cell lung cancer via miR-520a-5p/KRT5 axis.

Authors - Shan Chen
mouseIF

J Oral Biosci

Epigenetic modifier G9a is involved in regulation of mouse tongue development

Authors - Hisashi Ideno
humanIF

Cancer Lett

The Single Cell Immunogenomic Landscape after Neoadjuvant Immunotherapy combined Chemotherapy in Esophageal Squamous Cell Carcinoma

Authors - Zhe Yi Wang
humanIF

Bioact Mater

Biomimetic hydrogels with mesoscale collagen architecture for patient-derived tumor organoids culture

Authors - Jiaxin Wang
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