Cytokeratin 5 Polyclonal antibody
Cytokeratin 5 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IF-P, ELISA
Conjugate
Unconjugated
Cat no : 28506-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A431 cells, mouse skin tissues, rat skin tissues |
Positive IHC detected in | human tonsillitis tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human tonsillitis tissue |
Positive IF/ICC detected in | A431 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 1 publications below |
IF | See 5 publications below |
Product Information
28506-1-AP targets Cytokeratin 5 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Cytokeratin 5 fusion protein Ag25436 相同性解析による交差性が予測される生物種 |
Full Name | keratin 5 |
Calculated molecular weight | 590 aa, 62 kDa |
Observed molecular weight | 62 kDa |
GenBank accession number | BC024292 |
Gene symbol | Cytokeratin 5 |
Gene ID (NCBI) | 3852 |
RRID | AB_2881159 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
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WB protocol for Cytokeratin 5 antibody 28506-1-AP | Download protocol |
IHC protocol for Cytokeratin 5 antibody 28506-1-AP | Download protocol |
IF protocol for Cytokeratin 5 antibody 28506-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Invest Dermatol Circulating Exosomal miR-493-3p Affects Melanocyte Survival and Function by Regulating Epidermal Dopamine Concentration in Segmental Vitiligo. | ||
FASEB J Sperm immotility is associated with epididymis metabolism disorder in mice under obstructive azoospermia | ||
J Clin Lab Anal Hsa_circRNA_0017620 regulated cell progression of non-small-cell lung cancer via miR-520a-5p/KRT5 axis. | ||
J Oral Biosci Epigenetic modifier G9a is involved in regulation of mouse tongue development | ||
Cancer Lett The Single Cell Immunogenomic Landscape after Neoadjuvant Immunotherapy combined Chemotherapy in Esophageal Squamous Cell Carcinoma | ||
Bioact Mater Biomimetic hydrogels with mesoscale collagen architecture for patient-derived tumor organoids culture |