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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 647-conjugated Cytokeratin 5 Polyclonal antibody

Cytokeratin 5 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-28506

Synonyms

58 kDa cytokeratin, Cell and organelle markers, CK 5, CK5, Cytokeratin 5, Cytoskeleton Marker, K5, keratin 5, KRT5, KRT5A, Type II keratin Kb5

Validation Data Gallery

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  • Flow cytometry (FC) experiment of A431 cells using CoraLite® Plus 647-conjugated Cytokeratin 5 Polycl (CL647-28506)

    FC experiment of A431 using CL647-28506

    1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human Cytokeratin 5 (CL647-28506) (red), or 0.4 ug CoraLite® Plus 647-conjugated Rabbit IgG control Rabbit PolyAb (CL647-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive FC (Intra) detected inA431 cells
Positive FC detected inA431 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-28506 targets Cytokeratin 5 in FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cytokeratin 5 fusion protein Ag25436 相同性解析による交差性が予測される生物種
Full Name keratin 5
Calculated molecular weight 590 aa, 62 kDa
Observed molecular weight 62 kDa
GenBank accession numberBC024292
Gene symbol Cytokeratin 5
Gene ID (NCBI) 3852
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 Cytokeratin 5 antibody CL647-28506Download protocol
Standard Protocols
Click here to view our Standard Protocols