Validation Data Gallery
Tested Applications
| Positive IF-P detected in | human tonsillitis tissue |
| Positive IF/ICC detected in | A431 cells |
| Positive FC (Intra) detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.4 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL555-28506 targets Cytokeratin 5 in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag25436 Product name: Recombinant human KRT5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 6-60 aa of BC024292 Sequence: SVSFRSGGSRSFSTASAITPSVSRTSFTSVSRSGGGGGGGFGRVSLAGACGVGGY 相同性解析による交差性が予測される生物種 |
| Full Name | keratin 5 |
| Calculated molecular weight | 590 aa, 62 kDa |
| GenBank accession number | BC024292 |
| Gene Symbol | Cytokeratin 5 |
| Gene ID (NCBI) | 3852 |
| RRID | AB_2919650 |
| Conjugate | CoraLite®555 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 557 nm / 570 nm |
| 激发激光 | Green Laser (532 nm)/Yellow-Green Laser (561 nm) |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P13647 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Cytokeratin 5 (CK5) is a type II intermediate filament protein predominantly expressed in basal epithelial cells of various tissues, including the skin, breast, salivary glands, urinary tract, and upper aerodigestive tract. It is commonly found in specialized cell types such as squamous cells, basal cells, and myoepithelial cells. CK5 forms heterodimers with cytokeratin 14 and plays a crucial role in providing structural support to epithelial cells
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL555 Cytokeratin 5 antibody CL555-28506 | Download protocol |
| IF protocol for CL555 Cytokeratin 5 antibody CL555-28506 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |