- Featured Product
- KD/KO Validated
CoraLite® Plus 647-conjugated Desmin Polyclonal antibody
Desmin Polyclonal Antibody for IF-P, FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF-P, FC (Intra)
Conjugate
CoraLite® Plus 647 Fluorescent Dye
Cat no : CL647-16520
Synonyms
Validation Data Gallery
Tested Applications
Positive IF-P detected in | mouse heart tissue |
Positive FC (Intra) detected in | C2C12 cells |
Recommended dilution
Application | Dilution |
---|---|
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL647-16520 targets Desmin in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Desmin fusion protein Ag9742 相同性解析による交差性が予測される生物種 |
Full Name | desmin |
Calculated molecular weight | 470 aa, 54 kDa |
Observed molecular weight | 52-53 kDa |
GenBank accession number | BC032116 |
Gene symbol | Desmin |
Gene ID (NCBI) | 1674 |
RRID | AB_2920232 |
Conjugate | CoraLite® Plus 647 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 654 nm / 674 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Desmin is the main intermediate filament protein in skeletal and cardiac muscle cells and is essential for both the structural integrity and the survival of muscle cells. As an abundant muscle-specific protein, desmin has been widely used as a marker of muscle derived tumors. Anti-desmin is also valuable in the differential diagnosis of tumors of uncertain origin.
Protocols
Product Specific Protocols | |
---|---|
IF protocol for CL Plus 647 Desmin antibody CL647-16520 | Download protocol |
FC protocol for CL Plus 647 Desmin antibody CL647-16520 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |