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  • Featured Product
  • KD/KO Validated

Emerin Recombinant antibody

Emerin Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230089B12

Cat no : 82888-3-RR

Synonyms

EMD, STA, LEMD5, EDMD, 230089B12

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Emerin Recombinant antibody (82888-3-RR)

    WB analysis using 82888-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82888-3-RR (Emerin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using Emerin Recombinant antibody (82888-3-RR)

    WB analysis of HeLa using 82888-3-RR

    WB result of Emerin antibody (82888-3-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Emerin transfected HeLa cells.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Emerin Recombinant antibody (82888-3-RR)

    IHC staining of human stomach cancer using 82888-3-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 82888-3-RR (Emerin antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

  • Flow cytometry (FC) experiment of HeLa cells using Emerin Recombinant antibody (82888-3-RR)

    FC experiment of HeLa using 82888-3-RR

    1x10^6 HeLa cells were intracellularly stained with 0.2 ug EMD Recombinant antibody (82888-3-RR, Clone:230089B12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82888-3-RR

    Affinity and Kinetic Characterization of 82888-3-RR

    Biolayer interferometry (BLl) kinetic assays of 82888-3-RR against Human EMD were performed. The affinity constant is 11.1 nM.

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 82888-3-RR is Emerin Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Emerin fusion protein Ag0236 相同性解析による交差性が予測される生物種
Full Name emerin
Calculated molecular weight 34 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC000738
Gene symbol EMD
Gene ID (NCBI) 2010
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Emerin antibody 82888-3-RRDownload protocol
IHC protocol for Emerin antibody 82888-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols