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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 750-conjugated Emerin Recombinant antibody

Emerin Recombinant Antibody for WB, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra)

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

230089B12

Cat no : CL750-82888-3

Synonyms

EMD, STA, LEMD5, EDMD, 230089B12

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CoraLite® Plus 750-conjugated Emerin Recombinant a (CL750-82888-3)

    WB analysis using CL750-82888-3

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-82888-3 (Emerin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HEK-293T cells using CoraLite® Plus 750-conjugated Emerin Recombinant a (CL750-82888-3)

    FC experiment of HEK-293T using CL750-82888-3

    1x10^6 HEK-293T cells were intracellularly stained with 0.4 ug CoraLite® Plus 750-conjugated Emerin Recombinant antibody (CL750-82888-3, Clone:230089B12)(red), or 0.4 ug Isotype Control (CL750-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells
Positive FC (Intra) detected inHEK-293T cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL750-82888-3 is Emerin Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Emerin fusion protein Ag0236 相同性解析による交差性が予測される生物種
Full Name emerin
Calculated molecular weight 34 kDa
Observed molecular weight 30-35 kDa
GenBank accession numberBC000738
Gene symbol EMD
Gene ID (NCBI) 2010
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission maxima wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Emerin (Emery-Dreifuss muscular dystrophy) (EMD or EDMD), a serine-rich nuclear membrane protein, is a member of the nuclear lamina-associated protein family. EMD may mediate membrane anchorage to the cytoskeleton by stabilizing and promoting the formation of a nuclear actin cortical network. Defects in EMD gene are the cause of Emery-Dreifuss muscular dystrophy type 1 (EDMD1), a degenerative myopathy characterized by weakness and atrophy of muscle without involvement of the nervous system, early contractures of the elbows Achilles tendons and spine, and cardiomyopathy associated with cardiac conduction defects. EMD inhibits beta-catenin activity by preventing its accumulation in the nucleus and is involved in HIV-1 infection.

Protocols

Product Specific Protocols
WB protocol for CL Plus 750 Emerin antibody CL750-82888-3Download protocol
Standard Protocols
Click here to view our Standard Protocols