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CoraLite® Plus 750-conjugated Emerin Recombinant antibody
Emerin Recombinant Antibody for WB
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
WB
Conjugate
CoraLite® Plus 750 Fluorescent Dye
CloneNo.
230089B12
Cat no : CL750-82888-3
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
The immunogen of CL750-82888-3 is Emerin Fusion Protein expressed in E. coli.
| Tested Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Emerin fusion protein Ag0236 相同性解析による交差性が予測される生物種 |
| Full Name | emerin |
| Calculated molecular weight | 34 kDa |
| Observed molecular weight | 30-35 kDa |
| GenBank accession number | BC000738 |
| Gene symbol | EMD |
| Gene ID (NCBI) | 2010 |
| Conjugate | CoraLite® Plus 750 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 755 nm / 780 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Emerin (Emery-Dreifuss muscular dystrophy) (EMD or EDMD), a serine-rich nuclear membrane protein, is a member of the nuclear lamina-associated protein family. EMD may mediate membrane anchorage to the cytoskeleton by stabilizing and promoting the formation of a nuclear actin cortical network. Defects in EMD gene are the cause of Emery-Dreifuss muscular dystrophy type 1 (EDMD1), a degenerative myopathy characterized by weakness and atrophy of muscle without involvement of the nervous system, early contractures of the elbows Achilles tendons and spine, and cardiomyopathy associated with cardiac conduction defects. EMD inhibits beta-catenin activity by preventing its accumulation in the nucleus and is involved in HIV-1 infection.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 750 Emerin antibody CL750-82888-3 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
