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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®555-conjugated ERK1/2 Polyclonal antibody (CL555-51068)

    IF Staining of HeLa using CL555-51068

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®555 ERK1/2 antibody (CL555-51068) at dilution of 1:200.

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CoraLite®555-conjugated ERK1/2 Polyclonal antibody

ERK1/2 Polyclonal Antibody for IF/ICC
Cat No. CL555-51068

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

ERK, ERK 2, ERK2, ERT1, MAP kinase 1, MAP kinase 2, MAP kinase isoform p42, MAPK 1, MAPK 2, MAPK1, MAPK2, p38, p40, p41, p41mapk, p42 MAPK, P42MAPK, PRKM1, PRKM2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®555
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-51068 targets ERK1/2 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase 1
Calculated molecular weight 360 aa, 41 kDa
Observed molecular weight 38-44 kDa
GenBank accession numberBC017832
Gene Symbol ERK2
Gene ID (NCBI) 5594
RRIDAB_2919654
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP28482
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.

Protocols

Product Specific Protocols
IF protocol for CL555 ERK1/2 antibody CL555-51068Download protocol
Standard Protocols
Click here to view our Standard Protocols