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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 647-conjugated ERK1/2 Polyclonal an (CL647-51068)

    IF Staining of HeLa using CL647-51068

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 647 ERK1/2 antibody (CL647-51068) at dilution of 1:100.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 647-conjugated ERK1/2 Polyclonal an (CL647-51068)

    FC experiment of HeLa using CL647-51068

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human ERK1/2 (CL647-51068) (red), or 0.2 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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CoraLite® Plus 647-conjugated ERK1/2 Polyclonal antibody

ERK1/2 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL647-51068

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Synonyms

ERK2, MAPK1, ERT1, ERK-2, ERK 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 647
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-51068 targets ERK1/2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase 1
Calculated molecular weight 360 aa, 41 kDa
Observed molecular weight 38-44 kDa
GenBank accession numberBC017832
Gene Symbol ERK2
Gene ID (NCBI) 5594
RRIDAB_2934971
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP28482
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 ERK1/2 antibody CL647-51068Download protocol
FC protocol for CL Plus 647 ERK1/2 antibody CL647-51068Download protocol
Standard Protocols
Click here to view our Standard Protocols