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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated FBL Monoclonal antibody (CL594-66985)

    IF Staining of HepG2 using CL594-66985

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 FBL antibody (CL594-66985, Clone: 3A9E8 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite®594-conjugated FBL Monoclonal antibody (CL594-66985)

    FC experiment of HepG2 using CL594-66985

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human FBL (CL594-66985, Clone:3A9E8)(red), or 0.8 ug CoraLite®594 Mouse IgG1 Isotype Control (MOPC-21) (CL594-65124, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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CoraLite®594-conjugated FBL Monoclonal antibody

FBL Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-66985
Clone No.3A9E8

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Synonyms

FLRN, fibrillarin, FIB1, FIB, EC:2.1.1.-

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66985 targets FBL in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FBL fusion protein Ag8841 相同性解析による交差性が予測される生物種
Full Name fibrillarin
Calculated molecular weight 321 aa, 34 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC019260
Gene Symbol FBL
Gene ID (NCBI) 2091
RRIDAB_3673511
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP22087
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fibrillarin (Fbl) is an essential nucleolar protein that plays an essential role in ribosome biogenesis and more particularly in the methylation of ribosomal RNAs and rDNA histones. FBL is highly expressed in various cancers and correlates with poor survival outcomes in cancer patients,such as hepatocellular carcinoma(PMID: 33376525). Knockdown of FBL sensitizes tumor cells and xenografts to DNA crosslinking agents, and leads to homologous recombination-mediated DNA repair defects(PMID: 37489617).

Protocols

Product Specific Protocols
IF protocol for CL594 FBL antibody CL594-66985Download protocol
FC protocol for CL594 FBL antibody CL594-66985Download protocol
Standard Protocols
Click here to view our Standard Protocols