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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 647-conjugated FBL Monoclonal antibody

FBL Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

3A9E8

Cat no : CL647-66985

Synonyms

FLRN, fibrillarin, FIB1, FIB, EC:2.1.1.-

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 647-conjugated FBL Monoclonal antib (CL647-66985)

    IF Staining of HepG2 using CL647-66985

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 647 FBL antibody (CL647-66985, Clone: 3A9E8 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 647-conjugated FBL Monoclonal antib (CL647-66985)

    FC experiment of HepG2 using CL647-66985

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human FBL (CL647-66985, Clone:3A9E8)(red), or 0.4 ug CoraLite® Plus 647 Mouse IgG1 Isotype Control (MOPC-21) (CL647-65124, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-66985 targets FBL in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FBL fusion protein Ag8841 相同性解析による交差性が予測される生物種
Full Name fibrillarin
Calculated molecular weight 321 aa, 34 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC019260
Gene symbol FBL
Gene ID (NCBI) 2091
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fibrillarin (Fbl) is an essential nucleolar protein that plays an essential role in ribosome biogenesis and more particularly in the methylation of ribosomal RNAs and rDNA histones. FBL is highly expressed in various cancers and correlates with poor survival outcomes in cancer patients,such as hepatocellular carcinoma(PMID: 33376525). Knockdown of FBL sensitizes tumor cells and xenografts to DNA crosslinking agents, and leads to homologous recombination-mediated DNA repair defects(PMID: 37489617).

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 FBL antibody CL647-66985Download protocol
FC protocol for CL Plus 647 FBL antibody CL647-66985Download protocol
Standard Protocols
Click here to view our Standard Protocols