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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated FYN Monoclonal antibody

FYN Monoclonal Antibody for IF/ICC, IF-P, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IF-P, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1G4B1

Cat no : CL488-66606

Synonyms

Proto oncogene Syn, Proto oncogene c Fyn, p59-Fyn, p59 Fyn, EC:2.7.10.2

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CoraLite® Plus 488-conjugated FYN Monoclonal antib (CL488-66606)

    IF Staining of human tonsillitis using CL488-66606

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL488-66606 (FYN antibody) at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of Neuro-2a cells using CoraLite® Plus 488-conjugated FYN Monoclonal antib (CL488-66606)

    IF Staining of Neuro-2a using CL488-66606

    Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using CoraLite® Plus 488 FYN antibody (CL488-66606, Clone: 1G4B1 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated FYN Monoclonal antib (CL488-66606)

    FC experiment of HeLa using CL488-66606

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human FYN (CL488-66606, Clone:1G4B1) (red), or 0.2 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF-P detected inhuman tonsillitis tissue
Positive IF/ICC detected inNeuro-2a cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

CL488-66606 targets FYN in WB, IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FYN fusion protein Ag11127 相同性解析による交差性が予測される生物種
Full Name FYN oncogene related to SRC, FGR, YES
Calculated molecular weight537aa,61 kDa; 482aa,55 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC032496
Gene symbol FYN
Gene ID (NCBI) 2534
RRIDAB_2883360
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 FYN antibody CL488-66606Download protocol
FC protocol for CL Plus 488 FYN antibody CL488-66606Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Clin Invest

Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signaling.

Authors - Zhixiong Liu
View Article