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Validation Data Gallery

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  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 647-conjugated FYN Monoclonal antib (CL647-66606)

    FC experiment of HeLa using CL647-66606

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human FYN (CL647-66606, Clone:1G4B1) (red), or 0.2 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated FYN Monoclonal antibody

FYN Monoclonal Antibody for FC (Intra)
Cat No. CL647-66606
Clone No.1G4B1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

FC (Intra)

Synonyms

Proto oncogene Syn, Proto oncogene c Fyn, p59-Fyn, p59 Fyn, EC:2.7.10.2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 647
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-66606 targets FYN in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FYN fusion protein Ag11127 相同性解析による交差性が予測される生物種
Full Name FYN oncogene related to SRC, FGR, YES
Calculated molecular weight537aa,61 kDa; 482aa,55 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC032496
Gene Symbol FYN
Gene ID (NCBI) 2534
RRIDAB_2935063
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP06241
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FYN, also named as p59-Fyn and SLK, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. FYN is implicated in the control of cell growth. It plays a role in the regulation of intracellular calcium levels, with isoform 2 showing the greater ability to mobilize cytoplasmic calcium in comparison to isoform 1. FYN is required in brain development and mature brain function with important roles in the regulation of axon growth, axon guidance, and neurite extension. FYN blocks axon outgrowth and attraction induced by NTN1 by phosphorylating its receptor DDC.

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 FYN antibody CL647-66606Download protocol
Standard Protocols
Click here to view our Standard Protocols