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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated Follistatin Monoclonal ant (CL594-60060)

    IF Staining of HeLa using CL594-60060

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 Follistatin antibody (CL594-60060, Clone: 4E9B10 ) at dilution of 1:200.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

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  • KD/KO Validated

CoraLite®594-conjugated Follistatin Monoclonal antibody

Follistatin Monoclonal Antibody for IF/ICC
Cat No. CL594-60060
Clone No.4E9B10

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

IF/ICC

Synonyms

Activin binding protein, follistatin, FS, FST

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-60060 targets Follistatin in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Follistatin fusion protein Ag0775 相同性解析による交差性が予測される生物種
Full Name follistatin
Calculated molecular weight 33 aa, 7 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC004107
Gene Symbol Follistatin
Gene ID (NCBI) 10468
RRIDAB_2883413
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodCaprylic Acid-Ammonium Sulfate Precipitation
UNIPROT IDP19883
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Follistatin (FST) is a member of the tissue growth factor β family and is a secreted glycoprotein that antagonizes many members of the family, including activin A, growth differentiation factor11 , and myostatin. It binds activin A with high affinity and whose expression can be induced by activin A and several other pro-inflammatory cytokines. Activin A-follistatin complexes are biologically inactive and bind to cell surface heparan sulphate-containing proteoglycans for internalisation and degradation. It has also been found to play a significant role in the management of skeletal muscle size and mass. It also has important roles in early embryonic development, differentiation of ovarian granulosa cells, liver fibrosis and polycystic ovarian syndrome.

Protocols

Product Specific Protocols
IF protocol for CL594 Follistatin antibody CL594-60060Download protocol
Standard Protocols
Click here to view our Standard Protocols