ChromoTek GFP-Booster ATTO488
anti-GFP VHH/ Nanobody conjugated to a fluorescent dye for IF/ microscopy.
Specificity
GFP and GFP derivates
Applications
IF, IHC, ICC
Conjugate
ATTO 488
Host
Alpaca
206
Cat no : gba488
Synonyms
Validation Data Gallery
Product Information
anti-GFP VHH/ Nanobody conjugated to a fluorescent dye for IF/ microscopy.
| Description | The GFP-Booster stabilizes, enhances, and reactivates the signal of GFP-fusion proteins. Due to its small size, the GFP-Booster enables higher image quality in epifluorescence, confocal, and super-resolution microscopy:
• Considerably higher tissue penetration rates • Superior accessibility and labelling of epitopes in crowded cellular/organelle environments • Less than 2 nm epitope-label displacement minimizes linkage error • Monovalent VHHs do not cluster their epitopes • Validation: structure and function characterized • Consistent and reliable performance due to recombinant production |
| Applications | IF, IHC, ICC |
| Host | Alpaca |
| Specificity/Target | CFP, AcGFP, EGFP, GFP, GFP S65T, mClover, EGFP A206K, pHluorin, PA-GFP, sfGFP, TagGFP, TagGFP2, Citrine, Ecitrine, EYFP, Venus, YFP, Ypet For the complete list, please click here: Fluorescent protein specificity table Nano-Booster |
| Conjugate | ATTO 488 |
| Physical State | Liquid |
| Suggested Dilution | IF/ICC/IHC: 1:200 |
| Type | Nanobody |
| Class | Recombinant |
| RRID | AB_2631386 |
| Affinity (KD) | Dissociation constant KD of 1 pM |
| Storage Buffer | 10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09% sodium azide |
| Storage Condition | Shipped at ambient temperature. Store at -20°C/-4°F. Avoid freeze-thaw cycles. Aliquot upon arrival. Protect from light. Stable for 6 months. |
| Note | This product is for research use only, not for diagnostic or therapeutic use |
Documentation
| SDS |
|---|
| SDS GFP-Booster ATTO488 (PDF) |
| Protocol |
|---|
| gba488 Datasheet GFP-Booster ATTO488 |
| GFP-TRAP Specificity |
|---|
| Fluorescent protein specificity table Nano-Booster |
| Brochure |
|---|
| Chromotek nanobodies brochure (PDF) |
Publications
| Application | Title |
|---|---|
Nature Effector T-cell trafficking between the leptomeninges and the cerebrospinal fluid. | |
Science Translation. An RNA biosensor for imaging the first round of translation from single cells to living animals. | |
Cell Dynamic Architecture of DNA Repair Complexes and the Synaptonemal Complex at Sites of Meiotic Recombination. | |
Cell Oxidative Metabolism Drives Immortalization of Neural Stem Cells during Tumorigenesis. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Tom (Verified Customer) (11-02-2023) | Used at 1:200 in 4% PFA fixed samples to enhance signal for a low expression level fusion protein. Really good increase in signal with minimal background/non-specific staining. Used succesfully at both 1 hour and 30 minute room temperature incubation
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FH Muthuraj (Verified Customer) (03-03-2023) | It was good, works excellent
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