ChromoTek GFP-Booster Alexa Fluor® 568

anti-GFP VHH/ Nanobody conjugated to a fluorescent dye for IF/ microscopy.

Specificity

GFP and GFP derivates

Applications

IF, IHC, ICC

Conjugate

Alexa Fluor® 568

Host

Alpaca

Cat no : gb2AF568

Synonyms

GFP tag, GFP, eGFP, eYFP, CFP, YFP, BFP, GFP-Booster, Green fluorescent protein, AcGFP, GFP S65T, mClover (Clover A260K), Monomeric EGFP A206K, pHluorin (ecliptic), PA-GFP, Superfolder GFP (sfGFP), TagGFP, TagGFP2, Citrine, Ecitrine, Venus, EYFP, Ypet



Product Information

anti-GFP VHH/ Nanobody conjugated to a fluorescent dye for IF/ microscopy.

DescriptionThe GFP-Booster stabilizes, enhances, and reactivates the signal of GFP-fusion proteins. Due to its small size, the GFP-Booster enables higher image quality in epifluorescence, confocal, and super-resolution microscopy

• Considerably higher tissue penetration rates

• Superior accessibility and labelling of epitopes in crowded cellular/organelle environments

• Less than 2 nm epitope-label displacement minimizes linkage error

• Monovalent VHHs do not cluster their epitopes

• Validation: structure and function characterized

• Consistent and reliable performance due to recombinant production

ApplicationsIF, IHC, ICC
HostAlpaca
Specificity/TargetCFP, AcGFP, EGFP, GFP, GFP S65T, mClover, EGFP A206K, pHluorin, PA-GFP, sfGFP, TagGFP, TagGFP2, Citrine, Ecitrine, EYFP, Venus, YFP, Ypet
For the complete list, please click here: Fluorescent protein specificity table Nano-Booster
ConjugateAlexa Fluor® 568
Physical StateLiquid
Suggested DilutionIF/ICC/IHC: 1:500- 1:1,000
Type Nanobody
ClassRecombinant
DOL2 fluorophores per Nanobody
RRIDAB_2827574
Affinity (KD) Dissociation constant KD of 1 pM
Storage Buffer10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA
Preservative: 0.09% Sodium azide
Storage ConditionShipped at ambient temperature. Store at -20°C/-4°F. Stable for 6 month. Protect from light.
NoteThis product is for research use only, not for diagnostic or therapeutic use

Publications

ApplicationTitle
IF

Nat Commun

Poly(ADP-ribose) binding to Chk1 at stalled replication forks is required for S-phase checkpoint activation.

Authors - WooKee Min
IF

Cancer Cell

An integrated cellular and molecular model of gastric neuroendocrine cancer evolution highlights therapeutic targets

Authors - Joscha Griger
IF

Nat Commun

HIV-1 diverts cortical actin for particle assembly and release

Authors - Rayane Dibsy

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Roxk (Verified Customer) (07-22-2022)

this is the second time that I purchase the GFP booster, first time i used the 488 and it worked great, this time I got the 568. Tissue used is colon and the two BoosterGFP worked differently, with the 568 having lots of trouble (lot issue???)

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:400
  • Cell Tissue Type: Mouse colon