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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CoraLite® Plus 488-conjugated GRP78/BIP Monoclonal (CL488-66574)

    WB analysis using CL488-66574

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66574 (GRP78/BIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  • KD/KO Validated

CoraLite® Plus 488-conjugated GRP78/BIP Monoclonal antibody

GRP78/BIP Monoclonal Antibody for WB
Cat No. CL488-66574
Clone No.1D6F7

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse

Applications

WB

Synonyms

BIP, FLJ26106, GRP 78, GRP78, Heat shock 70 kDa protein 5, HSPA5, MIF2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, HeLa cells, HepG2 cells, MCF-7 cells, NIH/3T3 cells, HSC-T6 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66574 targets GRP78/BIP in WB applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GRP78/BIP fusion protein Ag2188 相同性解析による交差性が予測される生物種
Full Name heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
Calculated molecular weight 78 kDa
Observed molecular weight78 kDa
GenBank accession numberBC020235
Gene Symbol GRP78/BIP
Gene ID (NCBI) 3309
RRIDAB_2919345
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP11021
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER).It is a constitutively expressed resident protein of the ER in all eukaryotic cells. Recently it has been reported that GRP78 is associated with apoptosis or inhibition of cancer cell growth.

Protocols

Product Specific Protocols
WB protocol for CL Plus 488 GRP78/BIP antibody CL488-66574Download protocol
Standard Protocols
Click here to view our Standard Protocols