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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated GRP78/BIP Monoclonal antibody

GRP78/BIP Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

1D6F7

Cat no : CL647-66574

Synonyms

BIP, FLJ26106, GRP 78, GRP78, GRP78/BIP, Heat shock 70 kDa protein 5, HSPA5, MIF2

Validation Data Gallery

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  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 647-conjugated GRP78/BIP Monoclonal (CL647-66574)

    FC experiment of HeLa using CL647-66574

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 647-conjugated GRP78/BIP Monoclonal antibody (CL647-66574, Clone:1D6F7)(red), or 0.4 ug CoraLite® Plus 647 Mouse IgG1 Isotype Control (MOPC-21) (CL647-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-66574 targets GRP78/BIP in FC (Intra) applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GRP78/BIP fusion protein Ag2188 相同性解析による交差性が予測される生物種
Full Name heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
Calculated molecular weight 78 kDa
Observed molecular weight 70-78 kDa
GenBank accession numberBC020235
Gene symbol GRP78/BIP
Gene ID (NCBI) 3309
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER).It is a constitutively expressed resident protein of the ER in all eukaryotic cells. Recently it has been reported that GRP78 is associated with apoptosis or inhibition of cancer cell growth.

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 GRP78/BIP antibody CL647-66574Download protocol
Standard Protocols
Click here to view our Standard Protocols