GSDMD Monoclonal antibody

GSDMD Monoclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

2E6C7

Cat no : 66387-1-Ig

Synonyms

DF5L, DFNA5L, FKSG10, gasdermin D, GSDMD, GSDMDC1


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Tested Applications

Positive WB detected inHEK-293 cells, A431 cells, COLO 320 cells, EC109 cells, HL-60 cells, human colon tissue, human small intestine tissue, K-562 cells, Raji cells, TF-1 cells, PC-3 cells, U2OS cells, A549 cells, HepG2 cells, Jurkat cells
Positive IP detected inTHP-1 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66387-1-Ig targets GSDMD in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen GSDMD fusion protein Ag16827 相同性解析による交差性が予測される生物種
Full Name gasdermin D
Calculated molecular weight 484 aa, 53 kDa
Observed molecular weight 47-53 kDa
GenBank accession numberBC008904
Gene symbol GSDMD
Gene ID (NCBI) 79792
RRIDAB_2881763
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GSDMD is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. GSDMD has been suggested to act as a tumor suppressor. Recently, GSDMD is identified as a new component of inflammasomes, and it is an executor of pyroptosis and required for interleukin-1β secretion. Cleavage of GSDMD by inflammatory caspases determines pyroptotic cell death.

Protocols

Product Specific Protocols
WB protocol for GSDMD antibody 66387-1-IgDownload protocol
IF protocol for GSDMD antibody 66387-1-IgDownload protocol
IP protocol for GSDMD antibody 66387-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Cancer

Metal-enriched HSP90 nanoinhibitor overcomes heat resistance in hyperthermic intraperitoneal chemotherapy used for peritoneal metastases

Authors - Qiang Wang
humanWB

PLoS Pathog

An NLRP3 inflammasome-triggered cytokine storm contributes to Streptococcal toxic shock-like syndrome (STSLS).

Authors - Lan Lin
humanWB

iScience

ASC/inflammasome-independent pyroptosis in ovarian cancer cells through translational augmentation of caspase-1

Authors - Ozlem Calbay
mouseWB

Biochem Pharmacol

Deficiency of purinergic P2X4 receptor alleviates experimental autoimmune hepatitis in mice

Authors - Zejin Liu
humanWB

Emerg Microbes Infect

Acquiring high expression of suilysin enable non-epidemic Streptococccus suis to cause Streptococcal toxic shock-like syndrome (STSLS) through NLRP3 inflammasome hyperactivation.

Authors - Lei Xu
mouseWB

Mol Med

MALAT1 knockdown alleviates the pyroptosis of microglias in diabetic cerebral ischemia via regulating STAT1 mediated NLRP3 transcription

Authors - Nan Zhao